An injectable bone substitute (IBS) made of a suspension of Calcium phosphate ceramic was used to filled dental root canal after removing of canal pulp. The aim of this study was to verify the ability of calcium phosphate ceramic suspension to fill the apical zone of teeth ex vivo (n=40) and in vivo in a sheep model (n=8). The results showed that injection is possible with a good level of BCP granules at the end of the root dental canal with extracted tooth. In vivo, the presence of blood pressure due to the pulpectomy is a negative parameter to allow a good filling. The scanning electron microscopy revealed mineral formation at the apex level with mineral tissue conduction between the BCP granules but only one tooth showed a good apical filling with a good sealing. The sealing of the apex seems to depend of the amount of BCP granules. Other experiments with other animal models closer to a Human model have to be performed before human trials.