The Effect Orthosilicic Acid on Collagen Type I, Alkaline Phosphatase and Osteocalcin mRNA Expression in Human Bone-Derived Osteoblasts In Vitro |
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| Journal | Key Engineering Materials (Volumes 309 - 311) |
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| Volume | Bioceramics 18 |
| Edited by | Takashi Nakamura, Kimihiro Yamashita and Masashi Neo |
| Pages | 121-124 |
| DOI | 10.4028/www.scientific.net/KEM.309-311.121 |
| Citation | Meera Q. Arumugam et al., 2006, Key Engineering Materials, 309-311, 121 |
| Online since | May, 2006 |
| Authors | Meera Q. Arumugam, D.C. Ireland, Roger A. Brooks, Neil Rushton, William Bonfield |
| Keywords | Alkaline Phosphatase, Collagen Type I, Human Osteoblast Cells, Orthosilicic Acid, Osteocalcin, Reverse Transcriptase-Polymerase Chain Reaction, Silicon |
| Abstract | The object of this study was to investigate the effect of the concentration of orthosilicic acid (0, 0.5, 1, 5 and 10µM) on gene expression in human osteoblast cells isolated from trabecular bone. This was measured using reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify messenger RNA (mRNA) levels for collagen type I, alkaline phosphatase and osteocalcin. Results showed that while collagen type I mRNA expression was increased by the addition of up to 10µM orthosilicic acid, ALP message was suppressed over time and osteocalcin levels were decreased. |
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