Paper Title:
Osteogenic Differentiation of Marrow Mesenchymal Stem Cells (MSCs) on Ceramic Total Joints - Significance of Patient's Serum for Proliferation and Differentiation of MSCs -
  Abstract

After culture expansion of mesenchymal stem cells (MSCs) from a few milliliter of fresh patient’s bone marrow, we applied the MSCs on alumina ceramic ankle prosthesis and further cultured in an osteogenic medium for 2 weeks. After the culture, the MSCs differentiated into osteoblasts, which fabricated bone matrix on the surface of ceramic prosthesis. The expansion of MSCs followed by osteogenic differentiation was done using the commercially available medium with some chemicals and patient’s own serum. The MSCs well proliferated and differentiated into osteoblasts, even the MSCs were from old aged (more than 70 years old) patients. The tissue engineered ceramic prostheses were implanted into osteoarthritic patients. Typical X-ray findings showed that radiodense areas began to appear around the cell-seeded areas on the prosthesis about 2 to 3 months after the operation. These findings confirmed the importance of tissue engineering approach for early bone fixation and the approach can be done using small number of bone marrow cells and patient’s own serum without adding animal-derived products.

  Info
Periodical
Key Engineering Materials (Volumes 309-311)
Main Theme
Edited by
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
Pages
1383-1386
DOI
10.4028/www.scientific.net/KEM.309-311.1383
Citation
H. Ohgushi, H. Machida, A. Oshima, N. Kotobuki, M. Hirose, A. Fujisawa, Y. Tanaka, Y. Takakura, "Osteogenic Differentiation of Marrow Mesenchymal Stem Cells (MSCs) on Ceramic Total Joints - Significance of Patient's Serum for Proliferation and Differentiation of MSCs - ", Key Engineering Materials, Vols. 309-311, pp. 1383-1386, 2006
Online since
May 2006
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Masahiko Inagaki, Yoshiyuki Yokogawa, Tetsuya Kameyama
451
Authors: Motohiro Hirose, Kazunori Hamada, Toshimitsu Tanaka, Noriko Kotobuki, Hajime Ohgushi, Shoji Yasuda, Tsutomu Furuzono
Abstract:The objective of this study was to evaluate cell adhesion and proliferation on the hydroxyapatite (HAp)-coated silk fibroin (SF) fabric....
923
Authors: Ayako Oyane, Koji Hyodo, Masaki Uchida, Yu Sogo, Atsuo Ito
Abstract:An ethylene-vinyl alcohol copolymer (EVOH) film with a laminin–apatite composite layer on its surface showed improved adhesion and...
1181
Authors: Tadashi Asahina, Insu Jeon, Kiyotaka Katou, Tsutomu Sonoda
Abstract:The cell-structure of highly porous aluminum material prepared by melt foaming technology was investigated under deformation with fine-focus...
1395
Authors: Hamdy F.M. Mohamed, Seiti Kuroda, Yoshinori Kobayashi, Bruno Tavernier, Ryoichi Suzuki, Akihiro Ohira
Chapter 2: Membranes
Abstract:Nafion® is one of the most popular proton conducting membranes for polymer electrolyte fuel cells (PEFCs). For the integration of Nafion® to...
57