Paper Title:
Effects of Serum Proteins in Apatite Layer Formed in Culture Medium on Initial Adhesion and Cell Proliferation
  Abstract

In cell culture medium containing serum proteins, a layer with a great role in ostelblast-like cell’s growth formed on a HAp ceramics by a coprecipitation with a deposition of a bone-like apatite and an adsorption of serum proteins. The adsorption of proteins is influential in the apatite deposition and the cell adhesion. A component of serum albumin is forming < 80% of total serum proteins, therefore we focused on the serum albumin. The serum albumin was removed from fetal bovine serum by using an albumin removal kit, and then the albumin free cell culture medium was prepared. The bone-like apatite layer was quickly formed on the HAp ceramics in the albumin free medium, however the high cell adhesion property was not confirmed. As a result of investigation of an initial cell adhesion, the initial cell adhesion property of the bone-like apatite layer was not excellent even though the layer contained the serum proteins. Moreover, the layer without serum proteins showed a meager cell adhesion property in every time of the cultivation. Therefore, it was revealed that the adsorption of the serum proteins only on the surface was not enough to the improvement of the cell growth. And a few days cultivation was required for an appearance of the remarkable effect on the cell growth on the layer formed by a coprecipitation reaction about the bone-like deposition apatite and the serum proteins adsorption.

  Info
Periodical
Key Engineering Materials (Volumes 309-311)
Main Theme
Edited by
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
Pages
283-288
DOI
10.4028/www.scientific.net/KEM.309-311.283
Citation
T. Kizuki, T. Saito, M. Ohgaki, Y. Yokogawa, "Effects of Serum Proteins in Apatite Layer Formed in Culture Medium on Initial Adhesion and Cell Proliferation", Key Engineering Materials, Vols. 309-311, pp. 283-288, 2006
Online since
May 2006
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$32.00
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