Grit-blasting using bioactive HA and biodegradable CMP followed by acid etching was done. The apatite formation of prepared Ti samples was evaluated by immersion in R-SBF. And cell viability, proliferation, and differentiation were conducted using MTT assay and ALP staining. In RSBF immersion tests, non-etched HA-blasted samples showed the faster apatite-like formation than other samples. Acid etched and non-etched HA-blasted samples showed better cell viability and proliferation compared to CMP-blasted samples after 1 and 3 days. And the cell differentiation of non-etched HA-blasted samples was better compared to etched ones, and etched and non-etched CMP-blasted samples.