The biosynthesis of low molecular weight bacterial cellulose (LMBC) by Static culture of Acetobacter xylinum HN001 has been investigated. The medium was prepared with coconut-water by adding sugar and N-compounds. Several conditions effected LMBC yields, such as start pH value of culture medium, culture temperature and culture time were investigated. In view of high yield of LMBC, the optimal culture time and culture temperature was about 70 hours and 32°C with yield of LMBC 1.2g/L. Molecular weight (MW) and molecular weight distribution (MWD) of LMBC were tested by GFC. Results showed that, though the start pH value of culture medium varied from 3.5 to 5.5, the MW of the product cellulose varied not notable and MWD of LMBC were all almost 1.3.