Paper Title:
Development of Nanophase β-Tricalcium Phosphate/Collagen Fiber Composites for Improving Cell Adhesion and Proliferation
  Abstract

A novel porous beta-tricalcium phosphate /collagen fibers (β-TCP/CF) composite, having a well-dispersed nano-sized β-TCP in collagen matrix, was developed by a wet-chemical method. The nano-composite was compared to conventional β-TCP on cytocompatibility by cell attachment, proliferation, alkaline phosphotatse (AKP) activity and scanning electron microscopy (SEM) analysis. These in vitro assays showed that the β-TCP/CF composite elicited cell adhesion and proliferation better then controls. Moreover experiments on osteoblast-like cells showed improved cell growth with the highly characterized nanophase structure. SEM micrographs also showed that the nano-sized composite exhibited much more viable cells in attachment on the surface compared with the controls. At 1, 3 and 5 days, AKP activity was not significant different for the tested and control samples, while at 7 day after culture, significantly increased AKP activity was observed for β-TCP/CF than for the control. The in vitro results obtained confirmed the remarkable improvement of cell adhesion and proliferation of the nano-sized β-TCP/CF composite, which may be a new promising candidate for tissue engineered bone substitute.

  Info
Periodical
Key Engineering Materials (Volumes 309-311)
Main Theme
Edited by
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
Pages
581-584
DOI
10.4028/www.scientific.net/KEM.309-311.581
Citation
B. G. Guan, W. Q. Yan, D. S. Yang, C. Zou, W. J. Weng, "Development of Nanophase β-Tricalcium Phosphate/Collagen Fiber Composites for Improving Cell Adhesion and Proliferation ", Key Engineering Materials, Vols. 309-311, pp. 581-584, 2006
Online since
May 2006
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$32.00
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