Paper Title:
Coprecipitation of Cell Adhesion Molecule with Calcium Phosphate on Hydroxyapatite Ceramic
  Abstract

Fibronectin (FN) and type I collagen (Col), which are kinds of extracelluar matrices, were coprecipitated with calcium phosphate to form a composite layer on a hydroxyapatite (HAP) ceramic using a supersaturated calcium phosphate solution (CP solution). The amounts of protein immobilized in the layers were determined to be 20.97±3.04 µg·cm-2 for FN, 5.26±0.19 µg·cm-2 for Col and 21.72±2.30 µg·cm-2 for simultaneously immobilized FN and Col. When osteoblastic MC3T3-E1 cells were cultured on the HAP ceramics with the composite layer containing FN and/or Col, calcified tissue was formed through the activity of the cells. The result showed that the composite layer accelerated the differentiation of MC3T3-E1 to bone-forming cells. It is assumed that osteoblastic cells in alveolar bone migrated and differentiated on the surface of the tooth roots when the artificial tooth roots were covered with the composite layer.

  Info
Periodical
Key Engineering Materials (Volumes 309-311)
Main Theme
Edited by
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
Pages
767-770
DOI
10.4028/www.scientific.net/KEM.309-311.767
Citation
Y. Sogo, Y. Ishikawa, N. Kondo, E. Uchimura, A. Oyane, T. Ohno, A. Ito, A. Yamazaki, "Coprecipitation of Cell Adhesion Molecule with Calcium Phosphate on Hydroxyapatite Ceramic ", Key Engineering Materials, Vols. 309-311, pp. 767-770, 2006
Online since
May 2006
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$32.00
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