The study considers an ultrasonic characterization on the thermal distribution in vicinity for a cylindrical thermal lesion formed in a biological tissue. The cylindrical heat source is made of a standard nichrome wire with the diameter of 1 mm. The wire was inserted inside a pork muscle housed in a cuboidal container made of perspex. The heat is conducted radially outwards from the wire to the surrounding tissue. Thermal distribution near the heated wire was predicted by numerically solving a bioheat transfer function using FemLab (Comsol, Inc.). As the wire temperature was raised from the environmental temperature 20 °C to more than 80 °C in steps of 5 °C, ultrasonic B-scan images were acquired at each temperature. We assessed the feasibility of detecting the lesion boundary using changes in echogenicity, changes in centroid frequency due to attenuation, tissue moving characteristics resulting from changes in the speed of sound, and elastograms. These observations will be of use in improving ultrasonic monitoring and guiding in HIFU surgery and thermo-therapeutic process in general.