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Detection of Listeria Monocytogenes Using an Automated Fiber-Optic Biosensor: RAPTOR

Journal Key Engineering Materials (Volumes 321 - 323)
Volume Advanced Nondestructive Evaluation I
Edited by Seung-Seok Lee, Joon Hyun Lee, Ik Keun Park, Sung-Jin Song, Man Yong Choi
Pages 1168-1171
DOI 10.4028/www.scientific.net/KEM.321-323.1168
Citation Gi Young Kim et al., 2006, Key Engineering Materials, 321-323, 1168
Online since October, 2006
Authors Gi Young Kim, Mark T. Morgan, Daniel Ess, Byoung Kwon Hahm, Aparna Kothapalli, Angela Valadez, Arun Bhunia
Keywords Biosensor, Fiber Optic, Food Safety, Listeria Monocytogenes
Abstract

Fiber-optic biosensor uses light transmittable tapered fiber to send excitation laser light and receive emitted fluorescent light. The fluorescent light excited by an evanescent wave generated by the laser is quantitatively related to biomolecules immobilized on the fiber surface [1]. An automated fiber-optic biosensor based detection method for Listeria monocytogenes was developed in this research. Detections of Listeria monocytogenes in hotdog sample were performed to evaluate the method. By using the detection method with automated fiber-optic biosensor, 5.4×107 cfu/ml of Listeria monocytogenes was able to detect.

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