Therefore the objective of this study was to examine the differences of calf temperature by infrared thermal imager between on anti-fatigue matt and on the floor without mat. The subjects were instructed to wear short pants. It was measured with bare feet because influence of shoes and socks was to except. Subjects worked during 1 hour on the mat or without mat and next day without mat or on the mat. The order and load of work kept regularly. In this study, there was also a significant difference (p < 0.001) in the calf skin temperature between conditions, and calf temperature was higher when the subjects were standing on the mat than when they were standing on the floor without mat. By providing an elastic surface to stand on, a matt simulates muscle activity in feet and legs promote blood circulation. As a cross-modality evaluation, electromyogram was measured. There was the significant difference in Median Frequency (p < 0.001) and Median Power Frequency (p < 0.05) of EMG between two conditions. Median Frequency and Median Power Frequency showed a tendency to more decrease on the floor.