In this study, the dynamic fatigue characteristics of chemical starch-based ER fluid are experimentally investigated. A flow mode type apparatus is manufactured to activate the flow motion of the ER fluid. After evaluating the field-dependent Bingham property, three important dynamic fatigue characteristics; yield stress, current density and response time of the ER fluid are investigated as a function of the operating cycle. The dynamic operation for the flow motions is undertaken up to one million cycles and electric voltage is applied to the electrodes. In addition, the change of the particles of the ER fluid is microscopically observed to advocate the variation of the yield stress.