Paper Title:
Novel Gene-Transferring System Using a Laminin-DNA-Apatite Composite Layer
  Abstract

A laminin–DNA–apatite composite layer was successfully formed on the surface of an ethylene–vinyl alcohol copolymer. The immobilized DNA was transferred to the cells adhering onto the laminin–DNA–apatite composite layer more efficiently than those adhering onto a lamininfree DNA–apatite composite layer. It is considered that laminin immobilized in the surface layer enhances cell adhesion and spreading, and DNA locally released from the layer is effectively transferred into the adhering cells, taking advantage of the large contact area. The present gene transferring system, which shows high efficiency and safety, would be useful in gene therapy and tissue engineering.

  Info
Periodical
Key Engineering Materials (Volumes 330-332)
Main Theme
Edited by
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Pages
1021-1025
DOI
10.4028/www.scientific.net/KEM.330-332.1021
Citation
A. Oyane, H. Tsurushima, A. Ito, "Novel Gene-Transferring System Using a Laminin-DNA-Apatite Composite Layer", Key Engineering Materials, Vols. 330-332, pp. 1021-1025, 2007
Online since
February 2007
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$32.00
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