Paper Title:
OPG and Rankl Expression in Osteoblasts Grown on Different HA Ceramics
  Abstract

Hydroxyapatite (HA) ceramics is one of the most widely used bone substitute in clinics. Limited information is available concerning how HA ceramics may affect osteoprotegerin (OPG) and RANKL expression. In this study, osteoblastic-like SaOS-2 cells were grown on HA ceramics sintered at different temperature for 3 and 6 days, RANKL and OPG mRNA expression were analyzed with quantitative in situ hybridization (QISH) technique. Result showed that SaOS-2 grown on HA ceramics sintered at 800°C expressed higher RANKL mRNA than on other two HA ceramics after 3 days’ culture. No significant difference in OPG expression on different surfaces was detected after 3 days and 6 days culture. This result suggests that HA sintered at low temperature tend to induce more bone remodeling after implantation.

  Info
Periodical
Key Engineering Materials (Volumes 330-332)
Main Theme
Edited by
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Pages
1095-1098
DOI
10.4028/www.scientific.net/KEM.330-332.1095
Citation
C. Y. Wang, Y. R. Duan, X. L. Wang, B. Markovic, J. Barbara, C. R. Howlett, H. Zreiqat, X. D. Zhang, "OPG and Rankl Expression in Osteoblasts Grown on Different HA Ceramics", Key Engineering Materials, Vols. 330-332, pp. 1095-1098, 2007
Online since
February 2007
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$32.00
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