Paper Title:
A Novel Collagen Sponge for Cartilage Tissue Engineering
  Abstract

A novel collagen sponge that can protect cell leakage during cell seeding was developed by wrapping all the surfaces except the upside of a collagen sponge with membrane that has pores smaller than cell. The collagen sponge was used for three-dimensional culture of human bone marrow-derived mesenchymal stem cells (MSCs). The cells adhered to the collagen, and proliferated to fill the spaces in the sponge. The cell seeding efficiency was higher than 95%. The MSCs cultured in the collagen sponge in the chondrogenic induction medium supplemented with TGF-β3 and BMP6 expressed genes encoding type II collagen, SOX9 and aggrecan. HE staining indicated the round morphology of differentiated cells and the extracelluler matrices were positively stained by safranin O and toluidine blue. Type II collagen and cartilage proteoglycan were detected by immunostaining with anti-type II collagen and anti-cartilage proteoglycan. These results suggest the chondrogenic differentiation of MSCs. The collagen sponge facilitated cell seeding and chondrogenic differentiation of MSCs, and will be useful for cartilage tissue engineering.

  Info
Periodical
Key Engineering Materials (Volumes 330-332)
Main Theme
Edited by
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Pages
1101-1104
DOI
10.4028/www.scientific.net/KEM.330-332.1101
Citation
G. P. Chen, D. Akahane, N. Kawazoe, Y. Shirasaki, M. Tanaka, K. Yamamoto, T. Tateishi, "A Novel Collagen Sponge for Cartilage Tissue Engineering", Key Engineering Materials, Vols. 330-332, pp. 1101-1104, 2007
Online since
February 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Tetsuya Tateishi, Guo Ping Chen
Abstract:A new method for the preparation of biodegradable porous scaffolds has been developed by using preprepared ice particulates as porogen...
59
Authors: Gang Wu, Ying Jun Wang, Hua Ding Lu, Xiao Feng Chen, Jian Dong Ye, Cheng Yun Ning, Na Ru Zhao
Abstract:In this article, a multilayer tissue engineering scaffold has been fabricated. The uppermost layer is consisted by the collagen and the...
1549
Authors: Teruaki Yoshida, Masanori Kikuchi, Yoshihisa Koyama, Kazuo Takakuda
Abstract:The purpose of this study is to analyze osteogenic activity of human osteoblastic MG63 cells on the bone-like self-organized...
317
Authors: Jiang Wu, Guang Hui Wang, Hong Zhang, Yu Ping Wu, Yang Cheng Lv, Jing Song Liu, Jie Ke Ma, Jiang Zhu
Abstract:In the present study, we have demonstrated that alginate and collagen sponge can act as scaffolds in order to support 3-dimensional structure...
1935
Authors: Francesca Gervaso, Francesca Scalera, Sanosh Kunjalukkal Padmanabhan, Antonio Licciulli, Daniela Deponti, Alessia Di Giancamillo, Cinzia Domeneghini, Giuseppe M. Peretti, Alessandro Sannino
Abstract:In this work a novel three-dimensional ostechondral substitute is proposed that is made of an inorganic/organic hybrid material, namely...
890