In this experiment, un-dried silver-hydroxyapatite-titania nanoparticles slurry was used to make membrane with Polyamide-66 by co-polymerization method. The morphology, chemical components and structures of the membrane were characterized by AAS, XRD, SEM and EDX. S. aureus, E. eoli, P.gingivalis, F.nucleatum and S.mutans were utilized to test the antibacterial effect. XRD results demonstrated that the membrane have characteristic diffraction peaks of pure HA. A homogeneous distribution of the Ca, P, Ti, and Ag element in the membrane was confirmed by EDX. Both surface and section showed porous structure which was confirmed by SEM and the holes size was average 20-30μm. The bacteria assay reflects to the antibacterial effect, 56.31 % of S. aureus and 50.10 % of E. eoli were killed after 24 hours incubation. However, 91.84 % of P. gingivalis, 90.64 % of F. nucleatum and 90.49 % of S. mutans were killed and pictures of SEM showed obviously fewer cells on the surface. So the nanocomposite membrane could be one of the bioactive materials with antibacterial properties for oral GBR technique.