Paper Title:
Improvement of the Adhesion and Proliferation of Human Osteoblastic-Like Cells with Biomimetic Nanoapatite Coatings
  Abstract

Biomimetic nanoapatite coatings was developed by functionally modified methods with a combination of topographic, chemical and biomimetic treatments on the surface of titanium (Ti) substrate. The biological behavior and bioactivity of functionally modified SLA implants with chemical and biomimetic treatments (SCB-treated Ti) using body like solution were investigated to compare with untreated Ti and SLA Ti plates as controls. The cell attachment, proliferation, alkaline phosphotatse (AKP) activity, cell morphology and differentiation were evaluated by using MTT, RT-PCR, scanning electron microscopy (SEM) and confocal laser-scanning microscope (CLSM) analysis system. The results showed that the cell adhesion and proliferation was enhanced on functionalized titanium surface with nano-scale apatite compared to the controls. SEM micrographs also revealed that the osteoblast-like cells spreadly grew along the surface. Cell morphology and differentiation could be further observed distinctly by CLSM graphs. Moreover, mRNA expression of alkaline phosphotatse in nucleus on the SCB-treated Ti increased obviously on the third day compared with the controls. The in vitro results demonstrated the remarkable improvement on cell adhesion and proliferation of the biomimetic nanoapatite on SCB-treated Ti, which could be used for orthopaedic/dental implants.

  Info
Periodical
Key Engineering Materials (Volumes 330-332)
Main Theme
Edited by
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Pages
385-388
DOI
10.4028/www.scientific.net/KEM.330-332.385
Citation
Z. J. Pan, B. G. Guan, D. S. Yang, J. Feng, W. Q. Yan, "Improvement of the Adhesion and Proliferation of Human Osteoblastic-Like Cells with Biomimetic Nanoapatite Coatings", Key Engineering Materials, Vols. 330-332, pp. 385-388, 2007
Online since
February 2007
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$32.00
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