GTR treatment alone has not led to predictable new periodontal tissue reconstruction. Recently, various types of tissue substitutes and alloplastic graft have been used in conjunction with the GTR technique. The aim of this study was to evaluate whether nano-hydroxyapatite/Polylactic acid (nHA/PLA) and bone-derived materials would be useful in the treatment of class II furcation, in order to provide an ideal bone replacement material for periodontal treatment. Mucoperiosteal flap was raised on the buccal aspects of the experimental teeth and class Ⅱ furcation defects were created surgically on premolars of each quadrant in 3 dogs. The defects were randomly repaired with nHA/PLA and collagenic membrane, partially deproteinized bone (PDPB) and collagenic membrane. Blank control group was stayed. Speciments obtained in 6 weeks were evaluated with general observation, histological microscopic analysis. The specimens of the implanted groups exhibited periodontal regeneration including newly formed cementum, periodontal ligament and new bone. The new-formed bone area in sites receiving nHA/PLA and PDPB combined with collagen membrane was greater than that in blank group.The new bone area in PDPB was smaller than that in nHA/PLA. nHA/PLA would be better alternative for periodontal regeneration.