DNA transfection by biomaterial is a promising strategy to stimulate cells in tissue engineering. Co-precipitation of Calcium Phosphate (CaP) with plasmid DNA (pDNA) is known for several decades as non-viral transfection agent . In this study, we report the co-precipitation of different pDNA into biomimetic calcium phosphate coating onto titanium alloy (Ti) plates in order to evaluate their efficiency of incorporation. And it is possible to tune the rate of coprecipitation by changing the volume of Calcium Phosphate Solution (CPS) and the [pDNA]. The structure of coating was affected by the presence of DNA in CPS solution.