Heating reduces the amount of Ti-OH groups in the surface layers that are considered to favor apatite nucleation. Thus, low temperature processes are developed to fabricate bioactive titania. Anatase/rutile dual layers were deposited on titanium substrates when they were soaked in TiOSO4/H2O2 solution at low temperature, and than aged in hot water, which can induce apatite formation in DMEM. The resulted titania layers have apatite-forming ability. The organic molecules in DMEM medium had reduced the apatite formation on as-treated substrates in some measure.