Paper Title:
Immobilization of Collagen on Hydroxyapatite and Its Interation with Cells
  Abstract

The adhesion of bone cells on substrate materials is generally measured by the removal torque and/or contact area between bone and implants. In this study, collagen was used as a surfacegrafting material on hydroxyapatite (HA) substrates to enhance the cell adhesion because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks (10mmΦ x 1mm) were prepared and then collagen was immobilbized on the HA surface using an 3-APTES coupling agent to improve the adhesiveness of cells on HA disk surfaces. NIH 3T3 fibroblasts were seeded on the collagengrafted and non-grated HA disks and cultured in a Dulbecco’s modified eagle medium containing 10% fetal bovine serum for 3 hrs to evaluate the cell adhesion on the HA samples. The fibroblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen.

  Info
Periodical
Key Engineering Materials (Volumes 330-332)
Main Theme
Edited by
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Pages
781-784
DOI
10.4028/www.scientific.net/KEM.330-332.781
Citation
K.H. Jung, I. K. Kang, S. M. Kim, M. W. Ahn, S. Y. Kim, "Immobilization of Collagen on Hydroxyapatite and Its Interation with Cells", Key Engineering Materials, Vols. 330-332, pp. 781-784, 2007
Online since
February 2007
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$32.00
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