O-doped Diamond-Like carbon (O-DLC) films were prepared by direct magnetic filtered cathodic vacuum arc deposition (DC-MFCVAD) under different O2 gas flow at room temperature (R.T.). Raman spectra were used to characterize structure of films. The wettabilities of the O-DLC films also were investigated by contact angle measurements using four common liquids. For studying films’ interaction with blood, platelet adhesion experiment in vitro was done to characterize anticoagulant property of the O- DLC films from an aspect. Furthermore, ultraviolet spectrophotometer was employed to measure the optical band gap of the synthesized films. All of the results showed that O-DLC films have good anticoagulant property, oxygen doping can prevent graphite-like tendency of DLC films and increase band gap in a way, this kind of film materials may be become a new candidated biomaterials.