Paper Title:
Bio-Engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion
  Abstract

Grafting of autologous iliac crest and decortication approach in posterior spinal fusion surgery has been the “gold standard”. However, the limited source of autograft has prompted extensive research into bone substitute and biological enhancement of the fusion mass. In this study, the application of stem cell therapy by tissue engineering method was investigated to enhance posterior spinal fusion with -tricalcium phosphate ceramics in rabbit model. Rabbit bone marrow derived mesenchymal stem cells were aspirated from trochanter region of proximal femur. The mesenchymal stem cells were grown and directed to differentiate into osteogenic cells by osteogenic supplement (ascorbic acid, -glycerophosphate and dexamethasone) in basal medium (10% FBS in DMEM). The osteogenic cells were seeded on tricalcium phosphate ceramics for one day (MSC group, n=6). The cell-ceramics composite was implanted onto autologous L5 and L6 transverse processes with decortication approach in posterior spinal fusion. The cell free ceramics acts as control (Control group, n=6) and iliac crest autograft as positive control (Autograft group). The spinal segments were harvested at week 7 post-operation. Manual palpation was performed with spinal segments to assess any movement of L5-L6 vertebral joint. The stiffness of the joint was considered as solid fusion. The specimens then were fixed by formalin and transferred to 70% ethanol. The BMC and volume of fusion transverse processes of L5 and L6 was measured by peripheral quantitative computed tomography. In manual palpation, 50% solid fusion was found in MSC group, 60% in autograft group but none in control group. Moreover, the BMC of L5 and L6 transverse processes in MSC group was greater than autograft and control group (45%, 40% respectively, p<0.01). The volume of transverse processes in MSC group was greater than autograft by 45% (p<0.01) and control group by 26% (p<0.05). In conclusion, the mesenchymal stem cells derived osteogenic cells augmented spinal fusion and bone mineralization.

  Info
Periodical
Key Engineering Materials (Volumes 334-335)
Edited by
J.K. Kim, D.Z. Wo, L.M. Zhou, H.T. Huang, K.T. Lau and M. Wang
Pages
1201-1204
DOI
10.4028/www.scientific.net/KEM.334-335.1201
Citation
C. W. Chan, L. Qin, K.M. Lee, H.Y. Yeung, Y. Y. Hu, K.H.K. Wong, R.C.L. Yip, J. C.Y. Cheng, "Bio-Engineered Mesenchymal Stem Cell-Tricalcium Phosphate Ceramics Composite Augmented Bone Regeneration in Posterior Spinal Fusion", Key Engineering Materials, Vols. 334-335, pp. 1201-1204, 2007
Online since
March 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: B. Niechoda, M. Gianoutsos, Yong Ning Yu, W.R. Walsh
663
Authors: H.Y. Yeung, J.Z. Liu, Ling Qin, Yun Yu Hu, Chang Wei Lu, K.M. Lee, Y.M. Chiu, Jack C.Y. Cheng
Abstract:Bone marrow mesenchymal stem cells (BMSCs) possess a high replicative capacity and have the capacity to differentiate into various connective...
39
Authors: Chan Wai Chan, K.H.K. Wong, K.M. Lee, Ling Qin, H.Y. Yeung, H.B. Fan, Yun Yu Hu, Jack C.Y. Cheng
Abstract:Basic fibroblast growth factor (bFGF) has been shown to maintain the osteogenicity of bone marrow derived mesenchymal stem cell (MSCs) in...
1137
Authors: Chan Wai Chan, K.M. Lee, Ling Qin, K.H.K. Wong, H.Y. Yeung, H.B. Fan, Yun Yu Hu, Jack C.Y. Cheng
Abstract:The limited source of autograft has prompted extensive research on bone substitute and biological enhancement of the fusion mass in spinal...
1149
Authors: Zhi Yu Zhou, Li Jin Zou, Hai Sheng Li, Cody Bunger, Xue Nong Zou
Abstract:An ideal bone graft material should have osteocondutive, osteroindurctive, and osteogenic features. Scientists and doctors have been trying...
1006