In this paper, we study the effect of repairing the dog's femoral defects with the artificial bone integrating the nano-calcium phosphates/zirconia porous artificial bone scaffold with the autologous osteoblasts. We transplanted the artificial bone to the femoral defect of the dog, and at the same time, simple scaffold and the autologous cancellous bone were implanted as the control group. 3 months after the transplantation, the specimen was taken out with complete integration with the bone in these 3 groups and the bone defect got the complete bone union. The mechanics strength test showed that the group of the artificial bone was the strongest, followed by the cancellous bone group, and the simple material group was lower, but the strength was stronger than that before the transplantation. In the sixth month, the complete femoral defect repair was found in each group and the complete formation of the Haversian canal can be found on the histology examination. According to the mechanics strength test, there was no significant difference (P<0.05).