The purpose of this study was to evaluate the bone interface response to different surface using bone remodeling rate (BRR) under cyclic loading. The tibiae of 10 New Zealand white rabbits were used. Each tibia of rabbits received 2 implants, one with anodic oxidation surface and the other with machined surface. Dynamic loading, a cyclic load of 100 N with 100 cycles was applied for 2 weeks. All implants were divided into 4 groups according to surface treatment and loading; (1) machined surface, unloaded, (2) anodic oxidation surface, unloaded, (3) machined surface, loaded, and (4) anodic oxidation surface, loaded. To determine the rate of bone formation, a series of fluorochrome bone labeling materials (tetracycline, alizarin red, and calcein green) were administrated intramuscularly at 10, 11, 12 weeks. To study the formation time of new bone, ground sections were examined under confocal laser scanning microscopy. Resonance frequency analysis (RFA) values were measured at the time of first surgery, second surgery and sacrifice. From this study, it was concluded that implants with anodic oxidation surface had higher value than machined surface on RFA measurement and anodic oxidation surface has more resistance to loading than machined surface.