Paper Title:
Hybrid Type of Tissue Engineered Biodisc Using Annulus Fibrosus Seeded PLGA Scaffold and Nucleus Pulposus Seeded MPEG-PCL Hydrogel: Preliminary Study
  Abstract

Recently, it has been studied tissue engineered technique as novel approaches for treatment of the degenerative intervertebral disc (IVD). We designed the hybrid type of IVD mimicked scaffolds with poly(lactide-co-glycolide) (PLGA) and methoxypoly(ethyleneglycol)- poly(-caprolactone) (MPEG-PCL) diblock copolymers in order to application for the tissue engineered IVD. The MPEG-PCL solutions formed a gel-to-sol phase transitions as the temperature was increasesd. MPEG-PCL diblock copolymers were prepared by ring opening polymerization, and then nucleus pulposus (NP) cell was impregnated. Also, in order to restore annulus fibrosus (AF), we fabricated PLGA scaffold by solvent casting/salt leaching method. We confirmed disc cell function in manufactured scaffold through MTT assay in vitro and gross morphology and special staining in vivo for the possibility of the application of tissue engineering techniques.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
173-176
DOI
10.4028/www.scientific.net/KEM.342-343.173
Citation
Y. K. Ko, S. H. Kim, H. J. Ha, S. J. Yoon, J. M. Rhee, M. S. Kim, H. B. Lee, G. S. Khang, "Hybrid Type of Tissue Engineered Biodisc Using Annulus Fibrosus Seeded PLGA Scaffold and Nucleus Pulposus Seeded MPEG-PCL Hydrogel: Preliminary Study", Key Engineering Materials, Vols. 342-343, pp. 173-176, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yong Ha Youn, Chun Ho Kim, Young Ju Choi, Yong Jae Gin, Young Sook Son
Abstract:The porous neutralized chitosan scaffold (NCS) was prepared by freeze-dry method. Its poor cell binding capacity was improved approximately...
185
Authors: Ho Yun Chung, Eun Jung Oh, Jin Hyun Choi, Byung Chae Cho
Abstract:Mesenchymal stem cells (MSCs) from bone marrow seem to be the one of best candidates to regenerate injured tissue. However, recent advances...
193
Authors: Mi Sook Kim, Yoon Jeong Choi, Gun Woo Kim, In Sup Noh, Yong Doo Park, Kyu Back Lee, In Sook Kim, Soon Jung Hwang
Abstract:In vitro cell behaviors of calvarial osteoblasts (MC3T3) were evaluated by seeding them on both the surface and inside of in situ hyaluronic...
765
Authors: Yun Cang Li, Jian Yu Xiong, C.S. Wong, Peter D. Hodgson, Cui E Wen
Abstract: In the present study, titanium (Ti) samples were surface-modified by titania (TiO2), silica (SiO2) and hydroxyapatite (HA) coatings using a...
67
Authors: Masanori Kikuchi
Abstract:International standard for test method on cell migration into a scaffold is one of the important things to evaluate the scaffold. The "cell...
836