The objective of this work was to investigate cell adhesion, Poly Glycolic Acid (PGA) and PGA/ collagen nano-fibers on the silicone membrane. PGA with the weight-mixing ration of 40% was fabricated through the electronspun technique. The behaviors of Human Umbilical Vein Endothelial cells on these scaffolds are evaluated. The highest cell adhesion was observed in the PGA/collagen fibers with the diameter of 500 nm. This study indicates the effect of nano-fibers on the Human Umbilical Vein Endothelial cells for better understanding of interactions of cells with scaffold materials. Such information will have important implications for implantable vascular tissue engineering constructs.