Paper Title:
Muscle-Derived Stem Cell/Polymer Hybrid Mixture for the Treatment of Urinary Incontinence
  Abstract

In this study, muscle-derived stem cell (MDSC)/Pluronics/polycaprolactone (PCL) microparticle hybrid mixture was prepared as a potential injectable urethral bulking agent for the treatment of urinary incontinence. The MDSCs were isolated from the gastrocnemius muscles of SD rats by a modified preplate method and characterized through FACS analysis using various primary antibodies (CD34, Sca-1, CD45 and desmin). The hybrid mixture was prepared by the mixing of PCL microparticles (diameter, 100~200 μm) and MDSCs-containing thermo-sensitive Pluronic (F127/F68 mixture) solution (4.5/5.5, w/v). The hybrid mixture was easily injected through 18G needle into the body and stably remained in the applied site without initial volume decrease, owing to a well-packed structure of PCL particles exhibited in the hybrid mixture. It was observed that the MDSCs were stably grown in the hybrid mixture without severe inflammation and immune reaction. From the results, we recognized that the hybrid mixture can be a good candidate as an injectable bulking agent for the treatment of urinary incontinence, due to their good injectability, volume retention and biocompatibility.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
281-284
DOI
10.4028/www.scientific.net/KEM.342-343.281
Citation
S. H. Oh, J. H. Kim, J. Y. Lee, S. H. Ghil, S. H. Yuk, J. H. Lee, "Muscle-Derived Stem Cell/Polymer Hybrid Mixture for the Treatment of Urinary Incontinence", Key Engineering Materials, Vols. 342-343, pp. 281-284, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Feng Jiang, Yang Li Zhao, Shuai Fu Chen, Rui Liang
Chapter 7: Pollution Control Project (Air, Water, Solid)
Abstract:Aerobic granular sludge (AGS) was cultivated successfully in a sequencing batch reactor (SBR), which has floc sludge as seeding sludge in...
1267
Authors: Zhi Li Chen, Wen Biao Wang, Li An Hou, Hong Xia Liu, Zhen Jie Ren
Chapter 12: Intelligent Building and Digitalized Urban Construction
Abstract:Under the same operating mode, the flux of non-bulking MBR(1#) descends much faster than the flux of filamentous bulking MBR(3#), mainly...
3809
Authors: Yu Jun Shen, Yue Qing Hou, Li Xin Zhao, Hai Bo Meng
Chapter 8: Waste Disposal and Recycling
Abstract:The influence of bulking agents on odor production in sludge composts was investigated. Three mixtures were composted over a 23-day period in...
1868