Paper Title:
Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells
  Abstract

Biodegradable porous poly(L-lactic acid) (PLLA) scaffolds were prepared using gas foaming method. The PLLA scaffolds with a hydrophobic surface were subjected to Ar plasma treatment and in situ acrylic acid (AA) grafting to obtain hydrophilic PLLA scaffold (PLLA-PAA). Cell-adhesive RGD peptide was then immobilized onto the AA-grafted PLLA (PLLA-PAA-RGD). Once rabbit bone marrow-derived mesenchymal stem cells (BM-MSC) were isolated, MSCs were seeded into PLLA control, PLLA-PAA, and PLLA-PAA-RGD scaffold and cultured for up to 4 weeks in chondrogenic medium with the addition of 10 ng/ml transforming growth factor (TGF)-β1. Surface analysis of AA-grafted PLLA identified significant alterations of surface characteristics, including reduced contact angle and different atomic compositions. From WST-1 assay at 4 weeks, cells were found more proliferative in PLLA-PAA than the others. Upon the histological analysis of Safranin O staining, chondrogenic differentiation of MSCs appeared to be progressed more actively in PLLA-PAA. The effect of RGD immobilization on MSC differentiation was barely notable.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
297-300
DOI
10.4028/www.scientific.net/KEM.342-343.297
Citation
H. J. Jung, K. D. Park, K. J. Cho, K. D. Ahn, D. J. Ahn, D. K. Han, "Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells", Key Engineering Materials, Vols. 342-343, pp. 297-300, 2007
Online since
July 2007
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Price
$35.00
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