Paper Title:
Laminin Immobilized on Titanium Oxide Films for Enhanced Human Umbilical Vein Endothelial Cell Adhesion and Growth
  Abstract

In prior work we have shown that titanium oxide (Ti-O) thin films have good blood compatibility. However, as well as being hemocompatible, biomaterials used in contact with blood should be cell compatible also. In the work described here, Ti-O films were synthesized using unbalanced magnetron sputtering (UBMS) and were modified by immobilizing laminin on the film surface for improving human umbilical vein endothelial cell (HUVEC) adhesion and growth. Scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy (FTIR) and contact-angle measurements were used to investigate the surface characteristics of the Ti-O films and the modified Ti-O films. The results suggest that Laminin can be biochemically immobilized on the Ti-O film surface. The modified layer of Laminin can improve the hydrophilicity and wettability of Ti-O films. In vitro HUVEC investigations reveal that Laminin immobilized on the film surface greatly enhances cell adhesion and growth on Ti-O films.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
305-308
DOI
10.4028/www.scientific.net/KEM.342-343.305
Citation
S.N. Ge, J. Y. Chen, Y. X. Leng, N. Huang, "Laminin Immobilized on Titanium Oxide Films for Enhanced Human Umbilical Vein Endothelial Cell Adhesion and Growth ", Key Engineering Materials, Vols. 342-343, pp. 305-308, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Jun Ying Chen, Yong Xiang Leng, Sh.N. Ge, Ping Yang, Jin Wang, H. Sun, Guo Jiang Wan, An Sha Zhao, Nan Huang
Abstract:In the work reported here, titanium oxide film were synthesized using magnetron sputtering from a high-purity Ti metal target while...
287
Authors: Jin Hyun Chung, Hye Ryeon Lim, Tek Hyung Lee, Mi Hee Lee, Yeon I Woo, Hyun Sook Baek, Seung Jin Lee, Jeong Koo Kim, Jong Chul Park
Abstract:Poly (D,L-latic-co-glycolic acid) (PLGA) has been used as the artificial scaffold for blood vessel formation. In order to hinder smooth...
93
Authors: Jing Lu, Thomas J. Webster
Abstract:Atherosclerosis, which is caused by endothelial dysfunction, vascular inflammation, and the build-up of lipids, cholesterol, calcium, and...
119
Authors: Qian Yu, Xin Mei, Jun Ying Chen, Nan Huang
Abstract:In this paper, the Ti-O films were prepared by unbalanced magnetron sputtering deposition system. The films were then treated by plasma...
1001
Authors: Shi Dong Hu, Bin En Nie, Jian Liang Zhou
Chapter 6: Biological, Macromolecule and Organic Functional Materials
Abstract:With the development of modern medical technology, the clinical range of biomaterial applications have gradually expanded. However,...
635