Paper Title:
Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype
  Abstract

Acknowledging the supportive influence of matrix molecules on the chondrocytic phenotype, we combined heparin and chitosan to develop a novel biomaterial, supporting chondrogenesis. Chitosan had been shown as a promising structural material for a number of tissue engineering applications. Similar to heparin, one of the glycosamino-glycans (GAGs) had been known to exert various influences on the biological activities. In this study we evaluated the potential of heparin/chitosan polyelectrolyte complex materials for controlling the proliferation and differentiation of chondrocytes. The heparin/chitosan polyelectrolyte complex was coated over the polyethyleneimine (PEI) layer precoated on the commercial polystyrene dish. When examined by using human chondrocyte cell line (C28/I2), the heparin/chitosan surfaces supported well not only the cell proliferation but also the chondrocytic functions, such as expression of collagen type II. These results indicated that the heparin/chitosan complex could be used to modulate the activity of chondrocytes in vitro.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
329-332
DOI
10.4028/www.scientific.net/KEM.342-343.329
Citation
H. J. Lee, K. H. Park, S. R. Park, B. H. Min, "Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype ", Key Engineering Materials, Vols. 342-343, pp. 329-332, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Anna Theocharidou, K. Tsoptsias, Eleana Kontonasaki, Lambrini Papadopoulou, C. Panayiotou, Konstantinos M. Paraskevopoulos, Petros Koidis
Abstract: Chitin is a polysaccharide abundant in nature. Its’ deacetylation product-chitosan- in combination with gelatin (collagen product) is...
866
Authors: Ai Wen Tan, Belinda Pingguan-Murphy, Roslina Ahmad, Sheikh Akbar
Chapter 1: Materials Research and its Applications
Abstract:In situ TiO2 nanofiber arrays have been successfully produced directly on a Ti-6Al-4V substrate by using thermal oxidation...
63
Authors: Ning Yang, Lu Shi, Yi Bin Guo, Hao Zhang, Li Chen
Chapter 6: Biomaterials
Abstract:Chitosan/Heparin (CS/Hep) modified temperature sensitive hydrogels of γ-PGA were prepared by layer-by-layer self-assembly process. The...
527
Authors: Hendita Nur Maulida, Fitriyatul Qulub, Azizah Fresia Rosdiani, Disca Sandyakala Purnama, Karina Dwi Saraswati, Prihartini Widiyanti
Abstract:Peripheral nerve injury with gaps between 5 and 30 mm can result in permanent paralysis because axons are cut. The distance between axons,...
78