Paper Title:
Optimization of Scaffold for a Successful Hydrogel-Seeding Method for Vascular Tissue Engineering
  Abstract

Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 μm, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
333-336
DOI
10.4028/www.scientific.net/KEM.342-343.333
Citation
I. S. Park, S. H. Kim, J. W. Han, Y. G. Ko, E. N. Chung, S. H. Kim, "Optimization of Scaffold for a Successful Hydrogel-Seeding Method for Vascular Tissue Engineering ", Key Engineering Materials, Vols. 342-343, pp. 333-336, 2007
Online since
July 2007
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Price
$32.00
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