Paper Title:

Glucosylated Polypropylenimine Dendrimer as a Novel Gene Carrier

Periodical Key Engineering Materials (Volumes 342 - 343)
Main Theme Advanced Biomaterials VII
Edited by Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages 457-460
DOI 10.4028/www.scientific.net/KEM.342-343.457
Citation You Kyoung Kim et al., 2007, Key Engineering Materials, 342-343, 457
Online since July, 2007
Authors You Kyoung Kim, In Kyu Park, Hu Lin Jiang, Rohidas B. Arote, Hwan Jeong Jeong, Eun Mi Kim, Myung Haing Cho, Hee Seung Bom, Chong Su Cho
Keywords Cytotoxicity, Gene Delivery, Glucosylation, Polypropylenimine (PPI) Dendrimer
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Abstract

Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micromethod. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.