Paper Title:
Glucosylated Polypropylenimine Dendrimer as a Novel Gene Carrier
  Abstract

Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micromethod. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
457-460
DOI
10.4028/www.scientific.net/KEM.342-343.457
Citation
Y. K. Kim, I. K. Park, H. L. Jiang, R. B. Arote, H. J. Jeong, E. M. Kim, M. H. Cho, H. S. Bom, C. S. Cho, "Glucosylated Polypropylenimine Dendrimer as a Novel Gene Carrier", Key Engineering Materials, Vols. 342-343, pp. 457-460, 2007
Online since
July 2007
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Price
$32.00
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