Glucosylated Polypropylenimine Dendrimer as a Novel Gene Carrier
| Periodical | Key Engineering Materials (Volumes 342 - 343) |
|---|---|
| Main Theme | Advanced Biomaterials VII |
| Edited by | Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon |
| Pages | 457-460 |
| DOI | 10.4028/www.scientific.net/KEM.342-343.457 |
| Citation | You Kyoung Kim et al., 2007, Key Engineering Materials, 342-343, 457 |
| Online since | July, 2007 |
| Authors | You Kyoung Kim, In Kyu Park, Hu Lin Jiang, Rohidas B. Arote, Hwan Jeong Jeong, Eun Mi Kim, Myung Haing Cho, Hee Seung Bom, Chong Su Cho |
| Keywords | Cytotoxicity, Gene Delivery, Glucosylation, Polypropylenimine (PPI) Dendrimer |
| Price | US$ 28,- |
Polypropylenimine (PPI) dendrimers have been used by many researchers as gene delivery carriers due to their high functionality. Glucose as a kind of carbohydrate is biocompatible and hydrophilic. In this study, we synthesized glucosylated PPI (G-PPI) dendrimers to reduce cytotoxicity. Glucose substitution of G-PPI dendrimers was determined by the sulfuric acid micromethod. The G-PPI dendrimer was complexed with plasmid DNA in various N/P ratios, and the complex was characterized. G-PPI dendrimers showed good DNA binding ability and high protection of DNA from nuclease attack. The G-PPI dendrimer had low cytotoxicity compared to PPI dendrimer by cytotoxicity assay. Also, transfection efficiency was influenced by glucosylation degree and the transfection efficiency for the G-PPI-5 was slightly higher than that of PPI dendrimer in HeLa cell line.