Paper Title:
The Double-Layered Microsphere: Encapsulation of Water-Soluble Protein with PLGA
  Abstract

The double-layered microspheres play an important role in controlling drug delivery for pharmaceutical application, because of the low initial burst compared with single-layered spheres and targetable delivery to specific organ. But it has drawback in loading drug and controlling size. In this study, we developed double-layered spheres using relatively simple oil-in-water (O/W) solvent evaporation method using bovine serum albumin (BSA) as water-soluble protein and poly(D,L-lactide-co-glycolide) (PLGA). BSA/PLGA double-layered microspheres were fabricated using O/W solvent evaporation method and investigated the specific character of double-layered microspheres according to the kind of surfactants. In SEM observation, double layered microsphere had spherical shape and smooth surface without pores. And the double layered microsphere using O/W solvent evaporation method was transparency because of slow evaporation of solvent. In fluorescent observation, we observed the fluorescent core in the double-walled spheres composed of FITC-BSA and PLGA using fluorescent observation. In the case of polyvinylalcohol as emulsifier, the yield was better than gelatin. As decreased concentration of PLGA, the size of double-layered microspheres deceased.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
513-516
DOI
10.4028/www.scientific.net/KEM.342-343.513
Citation
J. T. Ko, H. J. Jung, J. H. Mo, J. S. Cho, S. H. Yuk, H. S. Shin, M. S. Kim, J. M. Rhee, H. B. Lee, G. S. Khang, "The Double-Layered Microsphere: Encapsulation of Water-Soluble Protein with PLGA", Key Engineering Materials, Vols. 342-343, pp. 513-516, 2007
Online since
July 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Garnpimol C. Ritthidej, W. Pichayakorn, Chulalongkorn Kusonwiriyawong, V. Lipipun
Abstract:The purpose of this study was to prepare chitosan nanoparticles (CS NP) for controlled protein delivery. Two techniques, simple ionotropic...
751
Authors: Fan Li, Feng Tian, Chang Jun Liu
Abstract:Poly(3-hydroxybutyrate) (PHB) based microspheres were prepared via double emulsion solvent evaporation using polyethylene glycol (PEG),...
54
Authors: Chong Wang, Sze Nga Tong, Yuk Hang Tse, Min Wang
Chapter 1: Natural Fibre and Biocomposites
Abstract:Over the past decade, intensive research has been conducted on electrospinning of fibrous tissue engineering scaffolds and their applications...
118
Authors: Xin Zhang, Min Wang
Chapter 5: Processing and Manufacturing
Abstract:Electrospinning is a popular technique for producing micro-or nanofibers for diverse applications including filtration, catalysis, sensors,...
386
Authors: Xiao Mian Liu, Zong Hua Wang, Bing Yu, Hai Lin Cong, Xiu Ling Gao, Xin Yu Guo
Abstract:Polymethylmethacrylate (PMMA) modified poly(vinylidene fluoride) (PVDF) hollow fiber ultrafiltration membranes were prepared by wet spinning...
229