Paper Title:
A Novel Branch-Type PEGylation of Aptamer Therapeutics
  Abstract

We have developed a novel protocol to prepare a branch-type PEGylation for a long acting formulation of aptamer therapeutics. A symmetric doubler phosphoramidite and the following amine modified phosphoramidite synthon were introduced to the final sequence of antithrombin aptamer, as a model for various aptamer therapeutics. The conventional linear PEGylation reagents were conjugated to the terminal amine groups of oligonucleotide to prepare the branchtype PEGylated anti-thrombin DNA aptamer. The PEGylated aptamer exhibited the improved bioactivity to retard the occlusive thrombus formation in vitro.

  Info
Periodical
Key Engineering Materials (Volumes 342-343)
Edited by
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Pages
529-532
DOI
10.4028/www.scientific.net/KEM.342-343.529
Citation
H. G. Kang, J. K. Park, Y. B. Seu, S. K. Hahn, "A Novel Branch-Type PEGylation of Aptamer Therapeutics", Key Engineering Materials, Vols. 342-343, pp. 529-532, 2007
Online since
July 2007
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Price
$32.00
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