The aim of this study was to set up a SPR sensor and to investigate protein adsorption onto biomaterial surface using it. Methods: The adsorption of human albumin (Alb), fibrinogen (Fib) and immunoglobulin G (IgG) onto polyurethane (PU) H50-50 and Au surface were measured using this SPR sensor. Results: The results of protein adsorption showed that the amount of Alb, Fib and IgG adsorbed on PU (H50-50) are smaller than Au, and on these two material surfaces, the amount sequence of adsorbed protein is: IgG> Fib > Alb. Both the Alb/Fib ratio and Alb/IgG ratio on PU (H50-50) surface are higher than Au. Conclusion: The antithrombogenicity of PU (H50-50) is better than Au.