The microbiological influenced corrosion (MIC) behaviors of the ship plate steel directly exposed in different medias (the sterile seawater, the ferrous bacteria solution and the sulfate-reducing bacteria solution) were investigated with electrochemical impedance spectroscopy (EIS), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Corrosion potential, electrochemical impedance and micrographs of specimens under different experimental conditions were obtained. Results show that the FB and SRB in the marine environment affect the corrosion behavior of the ship plate steel greatly. The corrosion process in FB and SRB environment was controlled by both bacteria and corrosion products. The mechanism of MIC is discussed.