Specially designed SEM in-situ tensile and fatigue tests have been conducted to trace the entire process of crack initiation and propagation till fracture in an ultra-high strength steel MA250. TiN is a typical inclusion and its average size is in the range of 8~10μm in MA250 steel. The micro-mechanism of the effect of TiN inclusion on crack initiation and propagation at tensile and fatigue tests both have been studied in detail. Experimental results show the harmful effect of TiN on tensile and fatigue properties both. This work is helpful to establish the practical life prediction model for the characteristic inclusion parameters in ultra-high strength steel components. It also enlightens us to eliminate TiN in the further development of ultra-high strength steels.