Structural operating conditions may significantly differ from those applied during laboratory tests where the structure is well known, well installed and properly excited. For structures under their natural loading conditions, or excited by random forces, excitations cannot be measured and are usually non stationary. Hence, an improvement operational modal analysis is a useful complement to the traditional modal analysis approach. The aim of this paper is to present the application of a new identification procedure, named wavelet-based identification technique of structural modal parameters. Wavelet-based identification that works in time-frequency domain is used to identify the dynamic characteristics of the structural system in terms of natural frequencies, damping coefficients and mode shapes. The paper has shown how the amplitude and the phase of the wavelet transform of operational vibration measurements are related to eigenfrequencies and damping coefficients, and the wavelet-based spectrum analysis is used to identify the mode shapes of the structure. Those modal parameters can be used to detect damage of structures. A simulation example has demonstrated that current identified results are comparable with those previously obtained from the peak pick method in frequency domain and stochastic subspace identification in time domain.