Paper Title:
Osteoblastic Cell Differentiation on BMP-2 Pre-Adsorbed Octacalcium Phosphate and Hydroxyapatite
  Abstract

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4°C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4°C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
1025-1028
DOI
10.4028/www.scientific.net/KEM.361-363.1025
Citation
T. Kumagai, T. Anada, Y. Honda, M. Takami, R. Kamijyo, H. Shimauchi, O. Suzuki , "Osteoblastic Cell Differentiation on BMP-2 Pre-Adsorbed Octacalcium Phosphate and Hydroxyapatite", Key Engineering Materials, Vols. 361-363, pp. 1025-1028, 2008
Online since
November 2007
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$32.00
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