Paper Title:
Protein Adsorption on Hydroxyapatite Nano-Crystals with Quartz Crystal Microbalance Technique
  Abstract

Real time adsorption behaviors of six proteins with different isoelectric points on hydroxyapatite (HAp) nanocrystal surfaces have been investigated by using HAp sensors for quartz crystal microbalance with dissipation technique (QCM-D). The dissipation (D)–frequency (f) plots clearly showed that the different types of protein adsorption behaviors; the D-f plots of acidic proteins lie on one straight line with a constant slope under all initial protein concentrations, while those of neutral and basic proteins lie on two straight lines with different slopes. The acidic proteins formed a monolayer, while the neutral and basic proteins could cause conformational changes with the adsorbed amount of proteins. The QCM-D technique with novel HAp nanocrystal sensor is useful for the liquid phase changes of proteins on the surface.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
1119-1122
DOI
10.4028/www.scientific.net/KEM.361-363.1119
Citation
T. Yoshioka, T. Ikoma, A. Monkawa, T. Tonegawa, D. Chakarov, B. Kasemo, N. Hanagata, M. Tanaka, "Protein Adsorption on Hydroxyapatite Nano-Crystals with Quartz Crystal Microbalance Technique", Key Engineering Materials, Vols. 361-363, pp. 1119-1122, 2008
Online since
November 2007
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$32.00
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