Paper Title:
Isolation Yield and Osteogenic Potential of Human Bone Marrow Stromal Cells are Maintained Irrespective of Age or Gender
  Abstract

The relationships between donor age and gender and initial isolation yield and the osteogenic potentials of human bone marrow stromal cells (hBMSCs) have not been clearly elucidated. The authors investigated whether isolation yields and the osteogenic differentiation potentials of hBMSCs are indeed dependent on donor age or gender. Fresh bone marrow was aspirated from iliac crest of 72 donors (mean age 54.1 years; range, 23-84 years; 39 men and 33 women) undergoing total hip arthroplasty. Numbers of mononuclear cells, numbers of colony forming unit-fibroblasts (CFU-Fs) and alkaline phosphatase (ALP)-positive CFU-Fs, and numbers of BMSCs after isolation culture were not found to be significantly dependent on donor age or gender. Moreover, no significant age- or gender-related differences were observed in terms of the proliferation activities, ALP activities, and calcium contents of BMSCs during in vitro osteogenic differentiation. The data obtained from 72 human donors revealed no significant age- or genderrelated differences among hBMSCs in terms of isolation yields, proliferation activities, and osteogenic potentials.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
1149-1152
DOI
10.4028/www.scientific.net/KEM.361-363.1149
Citation
J. J. Yoo, J. H. Bang, K. H. Koo, K. S. Yoon, H. J. Kim, "Isolation Yield and Osteogenic Potential of Human Bone Marrow Stromal Cells are Maintained Irrespective of Age or Gender", Key Engineering Materials, Vols. 361-363, pp. 1149-1152, 2008
Online since
November 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Young Il Yang, Dong Lim Seol, S.J. Jeong, D.S. Lee, Won Hee Jang, S.S. Seo, Sung Jae Lee
Abstract:Fibrin is a natural polymer with excellent biocompatibility and widely used as a cell delivery vehicle in tissue engineering. However,...
35
Authors: Hee Jung Lee, You Young Jo, Sun Young Kook, Byoung Moo Seo, Pill Hoon Choung
Abstract:In this study, various dental stem cells were isolated and their mineralization ability was evaluated. First, we isolated postnatal stem...
129
Authors: S.J. Heo, S.E. Kim, Yong Taek Hyun, D.H. Kim, Hyang Mi Lee, Yeong Maw Hwang, S.A. Park, Jung Woog Shin
Abstract:This study evaluated the potential of the PCL (poly -caprolactone)/HA(Hydroxyapatite) composite materials as a scaffold for bone...
369
Authors: Takahisa Anada, Akihiro Araseki, Shou Matsukawa, Tomokazu Yamasaki, Shinji Kamakura, Osamu Suzuki
Abstract:Our previous studies suggested that synthetic octacalcium phosphate (OCP) enhances bone regeneration more than hydroxyapatite (HA). However,...
31
Authors: Sun Young Lee, Min Jung Son, Gil Son Khang, Young Suk Son, Chang Kuk You, Suk Young Kim, Hong In Shin, Eui Kyun Park, Shin Yoon Kim
Abstract:Recently, nanomaterials have received considerable attention because of their potential applications in the biomedical field. In the present...
1177