Paper Title:
Expression Patterns of Cell Surface Molecules on Human Bone Marrow Stromal Cells from Multi-Donors
  Abstract

The unique characteristics of cell surface molecules on human bone marrow stromal cells (hBMSCs) have not been clearly elucidated. The authors characterized 19 cell surface molecules on culture-expanded hBMSCs obtained from 10 human donors, by flow cytometry, calculated the averages and standard deviations of the expression frequencies of individual surface molecules, and evaluated their expression patterns with respect to donor-dependent variations. Surface molecules expressed at frequencies of more than 80% on cells included, CD49e, CD29, CD90, CD73, CD44, CD105, and CD146, those expressed at frequencies of 20-80% included, Stro-1, CD51, CD54, CD31, CD106, CD163, and CD34, whereas CD49d, CD14, CD144, CD11b, and CD45 were expressed at less than 20%. Substantial donor-dependent variations (>10% standard deviation in expression percentage) were observed in the expressions of CD105, Stro-1, CD51, CD54, CD31, CD106, CD163, and CD34. Our results demonstrate that CD49e, CD29, CD90, CD73, and CD44 can be used as a positive marker for hBMSCs, and that CD144, CD11b, and CD45 could be used as a highly selective negative marker. However, the expressions of CD54, CD31, and CD106 observed in the present study, contradicted prior reports and thus, their expressions remain controversial.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
1153-1156
DOI
10.4028/www.scientific.net/KEM.361-363.1153
Citation
J. J. Yoo, K. W. Nam, K. H. Koo, K. S. Yoon, H. J. Kim, "Expression Patterns of Cell Surface Molecules on Human Bone Marrow Stromal Cells from Multi-Donors", Key Engineering Materials, Vols. 361-363, pp. 1153-1156, 2008
Online since
November 2007
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