Paper Title:
Reconstruction of Liver Organoid Using an Apatite-Fiber Scaffold, a Radial-Flow Bioreactor and FCL-4 Cells of Hepatocyte Model
  Abstract

We have previously developed apatite-fiber scaffolds (AFSs) for bone tissue engineering using single-crystal apatite fibers and carbon beads. In the present investigation, we examined the possibility of reconstruction of a liver organoid via three-dimensional (3D) culture of hepatocytes using the AFSs and the radial-flow bioreactor (RFB), aiming to apply the scaffold as a matrix for regeneration of a real organ. FLC-4 cells were used as a model of hepatocyte. The cells were well-viable in the RFB settled with AFSs over a period for 28 d, compared with the cases of cellulose beads and apatite beads with high porosity of 85%. We conclude that the present AFS may be a promising scaffold for tissue engineering of liver.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
1165-1168
DOI
10.4028/www.scientific.net/KEM.361-363.1165
Citation
M. Aizawa, A. Hiramoto, H. Maehashi , T. Matsuura, "Reconstruction of Liver Organoid Using an Apatite-Fiber Scaffold, a Radial-Flow Bioreactor and FCL-4 Cells of Hepatocyte Model", Key Engineering Materials, Vols. 361-363, pp. 1165-1168, 2008
Online since
November 2007
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