Paper Title:
Stimulatory Effect of Nano-Sized Calcium Metaphosphate Particles on Proliferation and Osteoblastic Differentiation of Human Bone Marrow Mesenchymal Stem Cells
  Abstract

Recently, nanomaterials have received considerable attention because of their potential applications in the biomedical field. In the present study, we investigated the effects of nano-sized calcium metaphosphate (CMP) particles (50 nm) compared with micro-sized CMP particles (200-500 nm and 10 μm) on the proliferation and osteoblastic differentiation of human bone marrow stem cells (BMSCs). BMSCs were challenged with CMP particles with different sizes for 3, 5, and 7 days. An analysis of the proliferation revealed that the nano-sized CMP particles (50 nm) stimulated the proliferation of BMSCs up to 27.79% compared to the untreated control. This stimulatory effect of the nano-sized CMP particle was dose-dependent. CMP particles appeared to adhere on the surface of BMSCs but this did not cause distinguishable morphological changes. Moreover, all CMP particles (50 nm to 10 μm) were capable of stimulating an osteoblastic differentiation of BMSCs as accessed by alkaline phosphatase (ALP) and von Kossa stainings. Further molecular analysis revealed that all the CMP particles induced an expression of osteoblast-related genes such as osteocalcin (OC) and collagen I (Col I). Taken together, our data demonstrate that nano-sized CMP particles have the potential to stimulate the proliferation and osteoblastic differentiation of BMSCs.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
1177-1180
DOI
10.4028/www.scientific.net/KEM.361-363.1177
Citation
S. Y. Lee, M. J. Son, G. S. Khang, Y. S. Son, C. K. You, S. Y. Kim, H. I. Shin, E. K. Park, S. Y. Kim, "Stimulatory Effect of Nano-Sized Calcium Metaphosphate Particles on Proliferation and Osteoblastic Differentiation of Human Bone Marrow Mesenchymal Stem Cells", Key Engineering Materials, Vols. 361-363, pp. 1177-1180, 2008
Online since
November 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Hala Zreiqat, Georg Berger, Renate Gildenhaar, Christine Knabe
1059
Authors: Takao Saito, Hikoshiro Hayashi, K. Uoe, Takashi Kizuki, Kay Teraoka, Katsuya Kato, Yoshiyuki Yokogawa
Abstract:Our experiments of mouse osteoblast-like MC3T3-E1 cells cultured on a glass substrate showed that as surface roughness of a substrate...
573
Authors: D.H. Lee, Dong Wook Han, B.J. Park, Hyun Sook Baek, K. Takatori, M. Aihara, Kazufumi Tsubaki, Jong Chul Park
Abstract:b-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating...
241
Authors: Hee Jung Lee, You Young Jo, Sun Young Kook, Byoung Moo Seo, Pill Hoon Choung
Abstract:In this study, various dental stem cells were isolated and their mineralization ability was evaluated. First, we isolated postnatal stem...
129
Authors: Miho Nakamura, Akiko Nagai, Kimihiro Yamashita
VI. Cell Studies and Cell-Material Interactions
Abstract:The osteoblast behaviors on the biomaterial substrates are recognized to play a fundamental role in osteoconduction process. The purpose of...
357