Bone is a complicated heterogeneous, viscoelastic, anisotropic material. Its properties depend on the nature of mechanical forces in addition to the location as well as the age, sex and race of the subject. Human humerus bones retrieved from cadavers were selected and separated in to cortical and cancellous parts. They were heat treated at different temperatures 400°C, 600°C and 800°C respectively to study the crystalline nature and composition of cortical and cancellous region of the humerus bone separately. Hydroxyapatite (HAP) was present as the major phase in the bone powders at 600 and 800°C with well resolved peaks along with other mineral phases of calcium phosphate. No amorphous phases were present indicating that the crystal structure of calcium phosphate compounds is present in crystalline form. FTIR studies further confirm the presence of phosphate vibrational modes of the entire bone mineral along with the presence of carbonates and hydroxyl groups.