Paper Title:
Cellular Activity on Siloxane-Doped Poly(Lactic Acid)/Vaterite Composite Scaffolds
  Abstract

Cellular activities of human mesenchymal stem cells (MSCs) and osteoblasts (HOBs) on a silicon-releasable scaffold, which is siloxane-doped poly(lactic acid) / vaterite composite coated with hydroxycarbonate apatite (SPV-H), were estimated using two types of media, with or without organic factors, dexamethasone (Dex) and β-glycerophosphate (β-GP). The culture tests using MSCs shows that the level of alkaline phosphatase (ALP) activity in the cells cultured on SPV-H increased for 21-day culturing in medium without Dex and β-GP. The proliferation of MSCs on SPV-H was significantly higher than that on a poly(lactic acid) / vaterite composite coated with hydroxyapatite (PV-H) at all time points. In the case of supplementing Dex and β-GP to the medium, the level of ALP activity in MSCs cultured on SPV-H was higher in comparison with that on PV-H at all time points. Scanning electron microscopy showed that there were some agglomerates in HOBs cultured on the SPV-H surface after 21-day culturing in the medium without the factors, while there are no agglomerates on PV-H. The agglomerates were regarded from laser Raman spectroscopy as bone nodules. This result implies stimulation to HOBs by silicon species in SPV-H. SPV-H is expected to be useful as the scaffold for bone tissue engineering.

  Info
Periodical
Key Engineering Materials (Volumes 361-363)
Main Theme
Edited by
Guy Daculsi and Pierre Layrolle
Pages
399-402
DOI
10.4028/www.scientific.net/KEM.361-363.399
Citation
A. Obata, T. Kasuga, "Cellular Activity on Siloxane-Doped Poly(Lactic Acid)/Vaterite Composite Scaffolds", Key Engineering Materials, Vols. 361-363, pp. 399-402, 2008
Online since
November 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: S. Bertazzo, Celso A. Bertran
Abstract:In this work, Scanning Electron Microscopy (SEM) has been used to determine the size and morphology of bone mineral crystals obtained from...
3
Authors: Wei Zhang, Richard Blackburn, Abbas Dehghani
Abstract:Nanocomposites were prepared by embedding carbon black and silica into phenolic resin matrix, which was supported by SEM and particle size...
1525
Authors: Amanda Abati Aguiar, Valter Ussui, Christiane Ribeiro, Marcos A. Scapin, Dolores Ribeiro Ricci, Nelson Batista de Lima
Abstract:Induction of an apatite-forming ability on a 100% Y2O3-ZrO2, 100% Al2O3, and 80/20 and 20/80 wt% of zirconia-alumina (Y2O3-ZrO2-Al2O3)...
697
Authors: Vladimir A. Popov, S.A. Tulupov, E.A. Skryleva, Andrey L. Chuvilin, Ute Kaiser, Igor I. Khodos, M.N. Kovalchuk
Abstract:Copper matrix nanocomposites (materials for coatings) and reinforcements from nanodiamonds, nanosilica or diatomite were fabricated by...
220
Authors: Sheng Jun Lu, Zhi Min Zhou, Cai Hong Wang, Jie Yu
Chapter 2: Advanced Building Materials
Abstract:Microporous Poly (vinyl chloride) (PVC) was prepared by foaming of PVC powders using the solution of 2, 2’-azo-bis-iso-butyronitrile (AIBN)...
864