Paper Title:
A Novel Living-Cell Imaging System Design with Ultra-Long Working Distance and High Numerical Aperture
  Abstract

Microscopy is an important tool in biology and medicine, but it is often limited to optical imaging structures with high numerical aperture(NA) with a short working distance(wd), for example NA = 0.6 and wd <1 mm are usual. The common microscope objective is inadequate for imaging of living cells in culture as an optical imaging structure with both high numerical aperture and long working distance is required. In this study, a novel optical design has been developed to meet the long working distance and high resolution power imaging of living cells in a vessel with a high culture solution thickness, where cells need to be developed in about 48 hours or a week. The developed optical design was characterized by an ultra-long working distance (wd >13.5 mm) and high numerical aperture (NA = 0.7). This optical imaging system is not only good for the subcellular imaging of free-floating cells in culture, but also for the imaging of cells attached at a surface of vessel.

  Info
Periodical
Key Engineering Materials (Volumes 364-366)
Edited by
Guo Fan JIN, Wing Bun LEE, Chi Fai CHEUNG and Suet TO
Pages
1077-1082
DOI
10.4028/www.scientific.net/KEM.364-366.1077
Citation
G. L. Huang, Z. H. Dong, C. Deng, S. K. Xu, J. Zhu, X. Y. Yang, G. F. Jin, "A Novel Living-Cell Imaging System Design with Ultra-Long Working Distance and High Numerical Aperture ", Key Engineering Materials, Vols. 364-366, pp. 1077-1082, 2008
Online since
December 2007
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$32.00
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