Paper Title:
Novel Design of Confocal Fluorescence Detecting System for Capillary Array and Microchip DNA Fragment Analysis
  Abstract

A novel multi-channel DNA fragment analysis system is presented,which is based on single PMT confocal fluorescence detection and optical scanning adopting an f-theta lens. Capillary electrophoresis experiments were executed in the system for DNA marker pBR322/Hae III. Signal was processed with wavelet denoising. The system’s limit of detection (dsDNA with the probe of Thiazole Orange, TO) was evaluated to be 1.1841×10-11mol/L. Its working noise is much lower compared with that based on mechanical scanning one, and the stability and detecting sensitivity is high. The system was expected to be applied to both capillary array and microchip electrophoresis detection based on laser-induced fluorescence.

  Info
Periodical
Key Engineering Materials (Volumes 364-366)
Edited by
Guo Fan JIN, Wing Bun LEE, Chi Fai CHEUNG and Suet TO
Pages
1147-1150
DOI
10.4028/www.scientific.net/KEM.364-366.1147
Citation
H. Zheng, L. Q. Wang, Y. Shi, J. Wang, Z. K. Lu, "Novel Design of Confocal Fluorescence Detecting System for Capillary Array and Microchip DNA Fragment Analysis ", Key Engineering Materials, Vols. 364-366, pp. 1147-1150, 2008
Online since
December 2007
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Qian Xiang, Ying Gao
Chapter 12: Ecological Civilization and Low-Carbon Economy
Abstract:A capillary electrophoretic assay for determining synthetic antioxidant butylated hydroxyanisole in food has been developed. The extraction...
1855
Authors: Qian Xiang, Ying Gao, Feng Hui Xie
Chapter 12: Ecological Civilization and Low-Carbon Economy
Abstract:A sensitive determination method for pethidine based on capillary electrophoresis-electrochemiluminescence detection was described. Analysis...
1914
Authors: Qian Xiang, Ying Gao
Chapter 11: Environmental Protection and Economic Development
Abstract:A fast method for the separation and determination of the food additive propyl gallate has been established by using capillary...
1486
Authors: Hong Ping Li, Hong Li
Chapter 8: Advance in Engineering and Management
Abstract:Simulating the overlapping capillary electrophoresis spectrogram under the dissimilar conditions by the computer system , Choosing the...
1116
Authors: Chun Li Hao
Chapter 2: Engineering Research
Abstract:Here we studied the effects of plasma and blood on quantum dots by capillary electrophoresis. The fluorescence of QDs can be observed using...
233