Recently the polymeric LOC (lab-on-a-chip) has been developed by the bio technology and the micro electro mechanical system technology. It is possible that biological experiment and analysis is executed rapidly and easily using a simple specimen. Therefore, many researches on LOC are progressing actively in various fields such as medical, biotechnical, environmental aspects etc. Up to the present time, most of polymeric LOC has been manufactured by LIGA (lithograpie galvanofomung abformung) or chemical etching processes. However, these methods have several disadvantages which are the complicated fabrication stages, requirements for various special equipments and limitations of materials. In this study, in order to overcome these difficulties, the possibilities of micro fluidic channel features were executed by micro cutting process for the steel. So the micro mold insert with micro fluidic channel was designed and manufactured by micro cutting process for the injection molding.